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Application of Chromogenic In Situ Hybridization (CISH) for Human Papillomavirus (HPV) Genotyping

Ekaterina Kldiashvili and Sophio Bojgua

Background: The research aimed evaluation of the effectiveness of CISH for HPV genotyping on cervical smears and compare the results of genotyping with cytopathology findings of routine screening.

Methods and findings: Gynecological cytology cases (n=1000) were taken from the clinical laboratory. Cases were diagnosed routinely by application of liquid based cytology method. Patients with diagnosed atypia were recalled for obtaining of material for HPV genotyping. This has been performed by usage of CISH method.

Conclusion: CISH is effective and easy for implementation method for HPV genotyping on cervical smears. There has been revealed 92.2% concordance in average between genotyping results and cytopathology findings of routine screening.