International Journal of Drug Development and Research

  • ISSN: 0975-9344
  • Journal h-index: 49
  • Journal CiteScore: 11.20
  • Journal Impact Factor: 8.24
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Awards Nomination 20+ Million Readerbase
Indexed In
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Scimago
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Publons
  • MIAR
  • University Grants Commission
  • Euro Pub
  • Google Scholar
  • J-Gate
  • Secret Search Engine Labs
  • ResearchGate
  • International Committee of Medical Journal Editors (ICMJE)
Share This Page


Development and validation of first order derivative UV-Spectrophotometric method for determination of Sitagliptin in bulk and in Formulation

Jain Pritam, Chaudhari Amar, Desai Bhargav, Patel Shani, Patel Santsaran, Shimpi Hiren

Objective: A simple, rapid, accurate and economical First order UV-derivative spectrophotometric method has been developed for estimation of sitagliptin from bulk and pharmaceutical formulation. Materials and methods: The λmax of sitagliptin in methanol and water was found to be 267 nm. The same spectrum was derivatised in to first order derivative; showed maximum amplitude of the trough at 275 nm. The drug follows linearity in the concentration range 10-60 μg/ml with correlation coefficient value 0.998. Results: The proposed method was applied to pharmaceutical formulation and % amount of drug estimated 99.19 % was found in good agreement with the label claim. The accuracy of the method was checked by recovery experiment performed at three different levels i.e., 80%, 100% and 120 %. The % recovery was found to be in the range 98.54%– 99.98%. The low values of % R.S.D. are indicative of the accuracy and reproducibility of the method. The precision of the method was studied as an intra-day, inter-day variations and repeatability. The % R.S.D. value less than 2 indicate that the method is precise. Ruggedness of the proposed method was studied with the help of two analysts. Conclusion: The above method was a rapid and costeffective quality-control tool for routine analysis of sitagliptin in bulk and in pharmaceutical dosage form