Flyer

International Journal of Drug Development and Research

  • ISSN: 0975-9344
  • Journal h-index: 51
  • Journal CiteScore: 46.50
  • Journal Impact Factor: 26.99
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Awards Nomination 20+ Million Readerbase
Indexed In
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Scimago
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Publons
  • MIAR
  • University Grants Commission
  • Euro Pub
  • Google Scholar
  • J-Gate
  • SHERPA ROMEO
  • Secret Search Engine Labs
  • ResearchGate
  • International Committee of Medical Journal Editors (ICMJE)
Share This Page

Abstract

Development and Validation of Spectrophotometric method for determination of Methotrexate Incorporated into PLGA Implants

Cristina Magalhães Santos, Millene; Da Costa, Vivian Mara; Pereira, Adriana De Fátima; Silva-Cunha, Armando;Ligório Fialho, Sílvia ;Pereira Santinho Gomes, Ana Julia; Rodrigues Da Silva Gisele

A simple and reliable spectrophotometric method was developed and validated for the determination of methotrexate incorporated into PLGA implants and released from them. The detection of the drug was carried out in 0.1 ml/L hydrochloric acid at 307 nm. It provided specificity for the methotrexate in direct contact with the PLGA. The method was linear in the range of between 4 and 14 μg/mL presenting a good correlation coefficient (r = 0.99). The average accuracies of three concentrations ranged from 98.64 to 100.76 % and precision was close to 2%. The method showed robustness, remaining unaffected by deliberate variations in relevant parameters.The validated method can be successfully applied to determine methotrexate loaded PLGA implants.