Journal of Biomedical Sciences

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Effects of Glucosinolates Rich Broccoli extract against Triazophos induced Toxicity in Wistar Rats

Dharmender Sharma and Gurinder Kaur Sangha

Background: Triazophos (TZ), an organophosphate (OP) pesticide, is widely used as non-systemic broad spectrum pesticide against insect pests. OPs, apart from neurotoxicity and neurobehavioral changes in animals, have been shown to induce oxidative stress (OS) by generating elevated levels of reactive oxygen species (ROS) which culminates into oxidative stress (OS).

Methods: Present study was carried out to evaluate the efficacy of antioxidative potential of glucosinolates rich broccoli (Br) extract via three doses viz; 10, 20 and 30 μmol, against the toxic effects of 1/10th of LD50 of TZ dose and its reversal in female albino rats during 30 days experiment. TZ induced OS biomarkers of blood toxicity viz; catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GR), glutathione peroxidase (GPx), glutathione-S-transferase (GST), total antioxidant activity (AOA) and lipid peroxidation (LPO) were estimated.

Results: OS biomarkers such as CAT, SOD, GR and GPx were differentially modified in TZ treated female albino rats and were improved with broccoli supplementation alongwith TZ. The total antioxidant activity (AOA) was also significantly increased in plasma of all the treated rats (Br + TZ); while significantly reduced LPO levels were observed in all Br+TZ treated rats as compared to TZ treated rats.

Conclusion: Subtly improved CAT, SOD and GPx stress biomarkers alongwith reduced LPO levels and enhanced AOA suggest that the exposure of female albino rats to TZ may be reversed by antioxidant potential of broccoli extract