Molecular Enzymology and Drug Targets

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Evaluating the Impact of Boosting on-site Enzymes produced by Trichoderma reesei RUT C30 and Aspergillus saccharolyticus with Commercial Enzymes in lowering the use of Commercial Cellulases.

Vandana Rana, Birgitte K. Ahring

In-house production of cellulases from filamentous fungi is widely used, but their hydrolytic efficiency compared to commercial enzymes is limited. We studied the effect of supplementing in-house cellulases produced by Trichoderma reesei, RUT C30 and a novel strain, Aspergillus saccharolyticus with different types of commercial enzymes for the efficient hydrolysis of wet exploded loblolly pine. Cellic®Ctec 2, Cellic®Htec2 and Novozym 188 were used as the commercial base enzymes for supplementing the in-house produced enzymes. Compared to non- supplemented in-house enzymes preparation, commercial enzymes (Cellic®Ctec2) added in the same amount as FPU and CBU, resulted in 68% higher glucose yield using wet exploded loblolly pine (WELP) at a 20% DM concetration. The highest saccharifcation yield was achieved by supplementation of the in-house produced cellulases with Cellic®Htec2 compared to Cellic®Ctec2 and Novozym 188. Optimal glucose, xylose and mannose yields, 85%, 92% and 86%, respectively were achieved by using in-house enzymes (15 FPU/g cellulose) supplemented with commercial hemicellulase (7.5 FPU/g cellulose). These results showed that supplementing in-house enzymes with commercial enzymes can be advantageous and work for lowering the overall cost of enzymes in a biorefinery.