The goal of this review was to look for 5-methyl-cytosine and 5-hydroxymethylcytosine in the genome of the scavenger Daphnia pulex. First, the presence of 5-mC and 5-hmC in genomic DNA was demonstrated using antibodies specific for either 5-mC or 5-hmC. The presence of the two changes was then confirmed in selected areas of three qualities using sets of limitation proteins with varying aversion to methylation and hydroxymethylation (Cox4, Cand2 and Ephx1).
KeywordsDaphnia pulex; 5-methyl-cytosine; 5-hydroxymethyl-cytosine; Entire genome sequencing; epigenetic adjustments.
Published Date: 2022-10-28; Received Date: 2022-10-03
