Flyer

Annals of Clinical and Laboratory Research

  • Journal h-index: 14
  • Journal CiteScore: 2.64
  • Journal Impact Factor: 1.89
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Indexed In
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Directory of Research Journal Indexing (DRJI)
  • Publons
  • Euro Pub
  • Google Scholar
  • Secret Search Engine Labs
Share This Page

Abstract

Loop-Mediated Isothermal Amplification: A Rapid Tool for Microbial Diagnosis

Sheriff Agbaje*

The contributions of nucleic acid amplification to the field of medical science cannot be overemphasized. Polymerase Chain Reaction (PCR) has been the most preferred technique of nucleic acid amplification over the years as a result of its high sensitivity. However, PCR limitations which include high cost of set up and high level of expertise needed makes it a setback to many researchers. Loop Mediated Isothermal Amplification (LAMP) was introduced to fill these bridges. In this review, previous studies of LAMP are highlighted, laying more emphasis on its types, usage, detection of its amplicons as well as its merits and demerits. LAMP advantages include its simplicity, cost effectiveness, rapidity, specificity and direct use of sample from site of infection. The various application of LAMP is also discussed. We have concluded that Since LAMP conform to the criteria set by the World Health Organization for an ideal diagnostic tool, LAMP will continue to be a valuable and important tool for diagnostic and research both in developed and developing countries in the future.

Published Date: 2022-08-31; Received Date: 2022-08-05