International Journal of Drug Development and Research

  • ISSN: 0975-9344
  • Journal h-index: 49
  • Journal CiteScore: 11.20
  • Journal Impact Factor: 8.24
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Awards Nomination 20+ Million Readerbase
Indexed In
  • Genamics JournalSeek
  • China National Knowledge Infrastructure (CNKI)
  • CiteFactor
  • Scimago
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Publons
  • MIAR
  • University Grants Commission
  • Euro Pub
  • Google Scholar
  • J-Gate
  • Secret Search Engine Labs
  • ResearchGate
  • International Committee of Medical Journal Editors (ICMJE)
Share This Page


Methylytransferase Activity of Dengue RNA virus by Using Purified Protein

Afaf S. Alwabli*, Sana G. Alattas, Alawiah M. Alhebshi, Nidal M. Zabermawi , Naser Alkenani, Khalid Al-ghmady and Ishtiaq Qadri

Dengue disease (DENV) is the purpose behind dengue fever. It is a mosquito-borne single positive-stranded RNA contamination of the family Flaviviridae; sort Flavivirus. Dengue illness is a mosquito-borne making or re-rising pathogen. Its positive sense RNA genome has a top at the 5'- end and no poly(A) tail at the 3'- end. The viral RNA encodes a single polyprotein, C-M-E-NS1-NS2A-NS2B-NS3-NS4A-NS4B-NS5. The polyprotein is prepared into three auxiliary proteins (C, M, and E) and seven nonstructural (NS) proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B, NS5). NS3 and NS5 are multifunctional chemicals performing different undertakings in the viral life cycle. The N-terminal area of NS5 has unmistakable GTP and S-adenosylmethionine (SAM) restricting locales. The job of GTP restricting site is trapped in the guanylyltransferase (GTase) movement of NS5. We depict the development of the MTase space of NS5 in an E. coli articulation vector and conditions for enzymatic activity of N7-and 2'O-methyltransferase exercises that yield the last sort I 5'- topped RNA.