International Journal of Drug Development and Research

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Study of Gyrase-A Gene in Mycobacterium tuberculosis Complex and its Clinical Relevance

Neha Kukreti, Yusra Ahmad, Vijay Kumar, Narotam Sharma*, Satish Chandra Nautiyal

Objectives: This study is being done to create awareness among the public regarding the routine checkup and if any such disease is diagnosed, proper medication should be started on time to prevent condition of patients before reaching to critical situation.

Methods: A total of 70 patients were included in the study, for the determination of Mycobacterium tuberculosis (MTB) positive and for this mpb64 (Mannose-Binding Protein) gene was targeted at 240 base pair. The samples detected for MTB positive were taken for the determination of gyr-A gene resistance and then samples were undertaken to pre-amplification area for preparing master mix by using forward and reverse primer (5’-GATGACAGACACGACGTTGC-3’and 5’-GGGCTTCGGTGTACCTCAT-3’ respectively). After that samples were taken to thermal cycler PCR (Polymerase Chain Reaction) for amplification of gyr-A gene and then amplicons were taken for electrophoresis following the analyzing of amplicons in Electronic UV trans-illuminator system, in which gyr-A gene was targeted at 398 base pair to determine if sample is positive for fluoroquinolone resistance.

Results: Males were found highly positive for tuberculosis and gyrase-A resistance. Patients above 41 years were more prone to both tuberculosis and gyrase-A resistance.

Conclusions: Drug resistance is very common among the population which can only be prevented by creating awareness programs resistance.