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Visualising the Vasculature of a Human Glioma in Mouse Brain using CLARITY

Liu King Lun, Justyna Prystal, Nelofer Syed and Hei Ming Lai

Advancement of laser microscopy and molecular probing techniques in recent years has led to the development of various tissue clearing technologies to allow us to visualise a large piece of tissue in 3D. Using an adaptation of a novel tissue clearing technique, CLARITY, we were able to visualise vascularisation of tumour in a completely new dimension. This image is a colourcoded depth projection (z-stack depth = 618.76 m) of a piece of clarified mouse brain tissue with human glioma immunostained with CD31 antibodies, showing large penetrating branches of blood vessels at the tumour margin