Research Article - (2018) Volume 9, Issue 5
Min Ji Kang1, Yoon Sung Choi1,2, Jiyoung Lee3, Kyeong Seob Shin4, Young Uh5, Young Kwon Kim6, Hyunwoo Jin1,2* and Sunghyun Kim1,2*
1Department of Clinical Laboratory Science, College of Health Science, Catholic University of Pusan, Busan, Korea
2Clinical Trial Specialist Program for in vitro Diagnostics, Brain Busan 21 Plus Program, Graduate School, Catholic University of Pusan, Busan, Korea
3Department of Biomedical Laboratory Science, College of Health Sciences, Yonsei University, Wonju, Korea
4Department of Laboratory Medicine, Chungbuk National University College of Medicine, Cheongju, Korea
5Department of Laboratory Medicine, Yonsei University Wonju College of Medicine, Wonju, Korea
6Department of Biomedical Laboratory Science, College of Medical Sciences, Konyang University, Daejeon, Republic of Korea
Received date: August 28, 2018; Accepted date: November 2, 2018; Published date: November 27, 2018
Citation: Kang MJ, Choi YS, Lee J, Shin KS, Uh Y, et al. (2018) Genetic Variations of Candida glabrata Clinical Isolates from Korea using Multi-locus Sequence Typing. Arch Clin Microbiol Vol No:9 Iss No:4:86 DOI: 10.4172/1989-8436.100086
Copyright: © 2018 Kang MJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Background: Although Candida albicans is considered to be the major fungal pathogen of candidemia, severe infections by non-albicans Candida (NAC) spp. have been on the increase in recent years. Among NAC spp., C. glabrata has emerged as the second most common pathogen. Unlike other Candida spp., it is often resistant to various azole antifungal agents, such as fluconazole. However, few studies have been conducted to investigate its structure, epidemiology, and basic biology. Recently, multi-locus sequence typing (MLST) has been developed as a highly useful and portable molecular biology technique.
Methods: In the present study, MLST was performed with a total of 102 C. glabrata clinical isolates that were isolated from various types of clinical specimens. The present study was performed with a total of 102 C. glabrata clinical isolates that were isolated from various types of clinical specimens. The fungal internal transcribed spacer (ITS) gene wad amplified and sequenced to identify and confirm C. glabrata clinical isolates. For MLST, six housekeeping genes including 1,3-beta-glucan synthase (FKS), 3-isopropylmalate dehydrogenase (LEU2),myristoyl-CoA, protein Nmyristoyltransferase (NMT1), phosphoribosyl-anthranilate isomerase (TRP1), UTP-glucose-1-phosphate uridylyltransferase (UGP1), and orotidine-5'-phosphate decarboxylase (URA3) were amplified and sequenced. The results were analyzed by using the C. glabrata database.
Results: Of a total of 3,345 base-pair DNA sequences, 49 (1.5%) variable nucleotide sites were found and the results showed that a total of 12 different sequence types (STs) were identified from the 102 clinical isolates. As classified by STs, The ST138 was the most predominant sequence type (ST) in this study as a result of 52.9% (54/102), and the following most predominant ST was the ST63 as a result of 23.5% (24/102).
Conclusion: In conclusion, this data demonstrated that the ST138 was the most predominant ST in Korea. Further, we found eight undetermined STs (USTs) and then seven STs among these STs were given the number by PubMLST database. The data from this study might provide a fundamental database for further studies on C. glabrata, including its epidemiology, and evolution. Furthermore, the data might also contribute to the development of novel antifungal agents and diagnostic tests.
The article entitled “Genetic Variations of Candida glabrata Clinical Isolates from Korea using Multi-locus Sequence Typing” has been accepted for publication in the Archives of Clinical Microbiology considering the statements provided in the article as personal opinion of the author which was found not having any conflict or biasness towards anything. As the article was a perspective one, information provided by the author was considered as an opinion to be expressed through publication.
Publisher took decision to make the article online solely based on the reviewers suggestion which considered the article not but a personal opinion of the author. However, it is found that the author have some personal concerns and issues, therefore, being retracted from the journal.
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