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Niroj Man Amatya, MBCHB*

The effective management of Candiduria depends upon proper identification of Candida species and their antifungal susceptibility test. Therefore, this cross-sectional study is conducted to assess the occurrence of different Candida species in urine and determine their antifungal susceptibility pattern from productive age group 15- 65. The isolation and identification of Candida species from clean catch midstream urine were done by culturing on Sabouraud Dextrose Agar with chloramphenicol followed by germ tube test, sugar fermentation test, chlamydospore production test, and further differentiated by using CHROM agar. The antifungal susceptibility test was done on Muller Hinton Agar with 2% glucose and 0.5 µg/mL methylene blue, and result interpretation was done as recommended to CLSI guidelines (M 44-A2). Candida parapsilosis (14, 31.1%) and C. albicans (12, 26.67%) were the two most common isolates. From 586 urine samples, 45 Candida species were isolated in which the predominant one is C. parapsilosis (14, 31.1%) followed by C. albicans (12, 26.6%). The antifungal susceptibility test showed that fluconazole resistant (29.41%) was comparatively more than voriconazole (21.87%). Since non albicans Candida are emerging pathogen and its antifungal resistant pattern is hiking, it is crucial to incorporate fungus identification in routine testing and identify their antifungal susceptibility pattern to ensure effective treatment and monitor for any antifungal resistance.

Published Date: 2023-10-23; Received Date: 2023-09-21