Sandeep Kumar, Manila Yadav, Amita Yadav, Jaya Parkash Yadav
Background: Aloe vera is a plant of Liliaceae family has been traditionally used for a variety of medicinal purposes. It has multiple applications such as emollient, purgative, antibacterial, antioxidant, antifungal, antiseptic and in cosmetics industries. The aim of present study was standardization of HPLC protocol for quantification of aloe- emodin and study of antimicrobial activity of methanolic extracts of Aloe vera collected from different climatic regions of India against pathogenic reference ATCC strains.
Methods and findings: Antibacterial and antifungal activity was checked by agar well diffusion method. High Performance Liquid Chromatography was used for quantification of aloe- emodin from collected samples by comparing with standard aloe-emodin marker compound. The different accession of Aloe vera methanolic extracts revealed significant antimicrobial activity against the tested strains. Extracts were more active against gram negative bacteria than gram positive ones. Amounts of marker compound aloe- emodin concentration varied a lot in different accessions. Climatic conditions showed notable effect on quantity of marker compound.
Conclusion: The present study has showed the role of climatic conditions and geographical locations on the variation in amounts of aloe- emodin present in different crude extracts of Aloe vera due to presence of which it has showed strong antimicrobial activity.
