Archives of Clinical Microbiology

  • ISSN: 1989-8436
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Effects of gamma and electron beam irradiation on viability and DNA elimination of Staphylococcus aureus

Mona M. K. Shehata, Fatma Alzahraa M. Gomaa, Zeinab H. Helal

Owing to the inability of real-time polymerase chain reaction (PCR)to distinguish between signals originating from viable cells and DNA released fromdead cells, (i.e. it can detect dead cells) a risk exists that DNA from dead cells can resultin false-positive PCR signals, so it is important to use a proper method for sterilization to maintain the accuracy of the quantitative real-time PCR assay.