Journal of Neurology and Neuroscience

  • ISSN: 2171-6625
  • Journal h-index: 18
  • Journal CiteScore: 4.35
  • Journal Impact Factor: 3.75
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Awards Nomination 20+ Million Readerbase
Indexed In
  • Open J Gate
  • Genamics JournalSeek
  • The Global Impact Factor (GIF)
  • China National Knowledge Infrastructure (CNKI)
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Proquest Summons
  • Scientific Journal Impact Factor (SJIF)
  • Euro Pub
  • Google Scholar
  • Secret Search Engine Labs
Share This Page


Localization of P450 Aromatase in The Brain of Adult Catfish Heteropneustes fossilis and Regional Distribution of Estradiol-17�?Ž�?² and Testosterone: Gender and Seasonal Differences

Surabhi Mishra and Radha Chaube

P450 aromatase is the key enzyme that converts aromatizable androgens to estrogens. It is coded by the gene that exists in two forms: ovarian type and brain type. In the present study, we localized aromatase protein and gene (brain type) by immunocytochemical and in situ hybridization techniques, respectively, and the gene expression by RT-PCR. Aromatase immunoreactivity and transcript signals were observed in basophils of the proximal pars distalis of the pituitary, purported to be the gonadotrophs. The transcript signals were detected in the periventricular grey zone of optic tectum (PGZ), ventral telencephalic region, and radial glial cells lining the telencephalon, hypothalamus and olfactory bulb. Transcript levels of cyp19a1b showed sex, seasonal and regional differences. We measured testosterone (T) and estradiol-17β (E2) levels by specific ELISA kits. In the brain, T level is low in comparison to E2 level. Testosterone level is higher in postspawning phase and lower in spawning phase. The dimorphic expression varied with seasons, being higher in males in the preparatory and prespawning phases. E2 level is higher in female brain than in male brain especially in the preparatory and prespawning phases. E2 level is the highest in prespawning phase and lowest in postspawning phase. The steroids showed brain regional distribution with significant seasonal and dimorphic variations. Olfactory bulb has the highest T: E2 ratio in the resting, preparatory and prespawning phases in comparisons to other brain regions. All brain regions elicited a high level of E2, which showed dimorphic and seasonal variations, indicating high rate of aromatization. Differential levels of aromatase transcript, E2 and T in brain regions suggest a high level of neuroestrogen formation and E2 involvement in various neuroendocrine functions such as regulating spawning behaviour, plasticity, neurogenesis, etc.