Translational Biomedicine

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Low Molecular Weight Components from Various Sources Eliminate Oxidative Stress and Restore Physiological Characteristic of Animals at Early Stages of Cu- Induced Liver Fibrosis Development

Bozhkov AI, Nikitchenko YuV, Lebid KM, Ivanov EG, Kurguzova NI, Gayevoy SS, Sharko MO,Alsardia Mohammad MA and Al Begai Mohammad AY

Background: Low molecular weight components (proteins and peptides with a molecular weight less than 45 kDa), were isolated from bovine colostrum and Sacharomyces cerevisue and Pleurotus osteatus cell components. It has been determined their effects on physiological characteristics (weight change, body temperature, work capacity) and biochemical parameters (cholesterol, triacylglycerols, creatinine and albumin) and lipid hydroperoxides (figure prooxidant activity) activity of glutathione peroxidase (a measure of antioxidant system) on the model of Cu-induced liver fibrosis.

It is shown that irrespective of the method and the source of the LMWCC and the Mix-factor its components eliminated the loss of body weight, the body temperature normalized, and partially restored working capacity of animals with Cu-induced fibrosis.

Methods: A three-fold serial intraperitoneal administration of copper sulfate to animals at the dose of 33% of the lethal dose with an interval of 48 hours between injections was accompanied with development of liver fibrosis. Doses of "Mix-factor" and LMWCC were chosen for the removed of influence of experimental animal’s fibrosis. In the investigation were used the methods of definition of content of cholesterol, triacylglycerols, creatinine and albumin. Besides, it has been activity of glutathione peroxidase and content of lipid hydroperoxides.

Results: Content of cholesterol, triacylglycerols, creatinine and albumin remained unchanged in animals with Cuinduced liver fibrosis, a lipid hydroperoxides have increased by 90% in blood serum and 83% in liver mitochondria and activity of glutathione peroxidase was reduced.

Introduction of LMWCC and mix-factor increases the activity of glutathione peroxidase and normalize lipid hydroperoxides content. It has been identified S-shaped dose dependence of LMWCC on indicators of proantioxidant system.

Conclusion: The results showed that LMWCC obtained from various sources (from cow colostrum or from Pleurotus ostreatus fungi, or from yeast) activated antioxidant defense enzymes (glutathione peroxidase), eliminated oxidative stress in animals with Cu-induced liver fibrosis. These biochemical changes in the body of animals with fibrosis were accompanied by normalization of physiological characteristics (body temperature, growth rate of animals).