Hydrophilic interaction chromatography has increased interest in zwitterionic sulfobetaine-based monolithic stationary phases. Using methanol and tetrahydrofuran as the porogenic system, a new hydrophilic polymeric monolith was created in this study by photo-initiated copolymerization using pentaerythritol triacrylate. It is noteworthy that the time needed to construct this unique monolith was only 5 min, which was much less time than was needed to prepare previously described sulfobetaine-based monoliths using traditional thermally started copolymerization. Additionally, the shape, permeability, porosity, mechanical strength column efficiency, and repeatability of these monoliths were all good. Mechanistic studies suggested that the retention of polar analytes on the zwitterionic SBVI-based monolith may be caused by potent hydrophilic and electrostatic interactions. The resultant monolith in particular demonstrated high anti-protein adhesion capability and low broad protein adsorption. These outstanding characteristics appear to favour its use in bioanalysis. As a result, the novel zwitterionic sulfobetaine-based monolith was effectively used for the efficient enrichment of N-glycopeptides from complicated samples as well as the highly selective separation of tiny bioactive molecules.
KeywordsZwitterionic monolith; Sulfobetaine; Photo-initiated copolymerization; Hydrophilic interaction chromatography; Complex sample
Published Date: 2023-04-28; Received Date: 2023-04-03
