Archives of Clinical Microbiology

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Screening of Fibrinolytic Enzymes from Microorganisms Especially Actinomycetes from Different Biotopes in Manipur

Debananda S Ningthoujam and Sharmila Thokchom

Background: The present work was focused on screening fibrinolytic enzymes from actinomycete and bacterial strains obtained from different biotopes in Manipur.
Methods and Findings: The fibrinolytic enzyme activity of the isolates was screened on plasminogen-free fibrin plate. To determine the specific activity, the enzymes were partially purified through ammonium sulphate precipitation followed by dialysis. The stability of the enzymes at different pH and temperatures were also analyzed. A total of 33 isolates were screened for fibrinolytic activity, of which 12 showed positive results. Six strains (MBRL-575, NRB1-19, FS19, FS7, HA4 and SxL6), out of 12 fibrinolytic isolates, showed highly potent activity. MBRL 575 exhibited the highest enzyme activity of 33.66 U/ml, followed by NRB1-19, FS19, FS7, HA4 and SxL6 with specific activity of 33.48, 20.05, 19.38, 17.68 and 15.02 U/ml respectively. The enzymes were sTable at pH ranging from 5-9 and temperatures from -20 to 55°C.
Conclusion: The fibrinolytic enzymes can withstand alkaline pH and cold temperature. They are sTable at pH range of pH 5-9 and temperature ranging from -20 to 55°C.