Mahidol University, Thailand
Short Communication
Role of cholesterol transporters, ABCA1 and ABCG1 in cholangiocarcinoma
Author(s): Pattaya Seeree*
Statement of the Problem & Purpose: Epidemiology of cholangiocarcinoma (CCA) is high in Thailand and Southeast Asia. It is highly aggressive and poorly studied. In this investigation, the role of cholesterol transporters, ATP-binding cassette (ABC) A1 and ABCG1 are studied in HuCCA-1 cell line. ABCA1 and ABCG1 transporters are suspected to play a role in CCA lipid homeostasis.
Methodology: The expression and localization of ABCA1 and ABCG1 were investigated by western blot analysis and immunocytochemistry, respectively. The functions of ABCA1 and ABCG1 in CCA cells were examined out by cholesterol efflux assay to specific cholesterol acceptor and high-density lipoprotein (HDL). ABCG1 transporter was down regulated using siRNA interference. Cell phenotypic changes such as cell migration and cholesterol export ability were observed by woun.. View More»