Department of Biological Chemistry, University of Columbia, United States
Perspective
Single RNA Viruses in Translation and Replication Dynamics
Author(s): Vaishnavi Thakur*
Among the most common infections and a significant burden on society are RNA
viruses. Although RNA viruses have been extensively investigated, due to a lack
of sensitive assays, little is known about the processes that take place within
the initial few hours after infection. Here, we create the virus infection real-time
imaging (VIRIM) single-molecule imaging technique to examine the translation
and replication of individual RNA viruses in living cells. Virim revealed substantial
coordination between the translation and replication of individual viral RNAs as
well as a startling variability in replication kinetics between cells. Additionally,
we use VIRIM to pinpoint the host genes that prevent early viral replication and
identify the replication stage of the incoming viral RNA as a significant bottleneck
of effective infection. Single-molecule photograph.. View More»
DOI: 2572-5475- 09.01-121
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