C protein of PPRV inhibits the type I IFN signaling pathway

Peste des petits ruminants Virus (PPRV) is an important pathogen which causes a fatal disease to small ruminantsï¼�?such as goat and sheep. It threatens the economics of livestock industry, mainly in developing countries. In order to investigate PPRV C-mediated innate immune response, recombinant plasmids were first constructed to express the non-structural protein C. The results from a dual-specific luciferase reporter assay showed that PPRV C protein significantly inhibits retinoic acid-inducible gene I CARD (RIG-I N)-induced interferon-β (IFN-β), type I interferon (IFN) stimulated response element (ISRE) and nuclear factor kappa-B (NF-κB) activities. Subsequently, the results of qRT-PCR showed that the expression of IFN-β, and its downstream interferon stimulated gene 56 (ISG56) and ISG15, C-X-C motif chemokineï¼�?CXCL-10ï¼�?were down-regulated at the transcriptional levels, suggesting PPRV C protein inhibits the RIGI-mediated signalling pathway. Furthermore, the co-immunoprecipitation experiment was performed to test the interaction between PPRV C and key molecules in the RIG-I signalling pathway. The results indicated that PPRV C protein specifically interacts with mitochondrial antiviral signalling (MAVS) protein. In addition, PPRV C protein was co-transfected dose-dependently with RIG-I or MAVS into HEK293T cell. The Western blotting data showed that the high expression of PPRV C protein could inhibits the expression of MAVS protein, but not RIG-I. As conclusion, our results were demonstrated that the expression of PPRV C protein inhibits the activation of the IFN-β promote in cells which subsequently causes the virus to be more virulent. So, our study reveals that the target of PPRV C associated with the RLRs signalling pathway which can be used for eradication of PPRV worldwide.

Biography :

Linjie Li is the Postgraduate student in Northwest Minzu University, Lanzhou, China. She is specialised in animal molecular virology and molecular immunology

E-mail: 674482301@qq.com

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