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Archives of Clinical Microbiology

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Gentamicin enhances toxA expression in Pseudomonas aeruginosa isolated form cow mastitis

EuroSciCon Conference on Virology and Infectious Diseases
April 22-23, 2019 | Athens, Greece

Atheer Abdul Razzaq and Ansam KhAlid Mahmood

University of Baghdad, Iraq

Posters & Accepted Abstracts: Arch Clin Microbiol

Abstract:

The present study was undertaken in order to investigate the role of gentamicin in the gene expression of toxA in Pseudomonas aeruginosa isolated from cow mastitis. A total of ten P. aeruginosa strains originally isolated from cows infected with mastitis. Agar dilution methodology was performed to determine the minimal inhibitory concentration of gentamicin, all of which developed resistance toward gentamicin. The findings presented here demonstrated that all these strains harboured toxA depending on PCR-based assay. Nonetheless, RT-PCR technique revealed a wide variation in expression of toxA. Moreover, the cultivation of P. aeruginosa in the presence of gentamicin, significantly (P<0.05), induced the expression of toxA, in addition to the possibility of enhancing the virulence of this bacterium. In conclusion, using gentamicin to treat infections caused by P. aeruginosa may participate in more severe outcomes.

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