Flyer

Archives of Clinical Microbiology

  • ISSN: 1989-8436
  • Journal h-index: 24
  • Journal CiteScore: 8.01
  • Journal Impact Factor: 7.55
  • Average acceptance to publication time (5-7 days)
  • Average article processing time (30-45 days) Less than 5 volumes 30 days
    8 - 9 volumes 40 days
    10 and more volumes 45 days
Awards Nomination 20+ Million Readerbase
Indexed In
  • Open J Gate
  • Genamics JournalSeek
  • The Global Impact Factor (GIF)
  • Open Archive Initiative
  • China National Knowledge Infrastructure (CNKI)
  • Directory of Research Journal Indexing (DRJI)
  • OCLC- WorldCat
  • Proquest Summons
  • Publons
  • MIAR
  • University Grants Commission
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • Google Scholar
  • Scimago Journal Ranking
  • Secret Search Engine Labs
  • ResearchGate
  • International Committee of Medical Journal Editors (ICMJE)
Share This Page

Grayscale box counting for texture analysis in liver of common carp (Cyprinus carpio ) sub-chronically exposed to perfluorooctanoic acid (PFOA)

Joint Event on 3rd International Confrence on Digital Pathology & 7th Global Summit on Microbiology Research
August 13-14, 2018 Madrid, Spain

Maurizio Manera, Bahram Sayyaf Dezfuli, Giuseppe Castaldelli and Luisa Giari

University of Teramo, Italy University of Ferrara, Italy

Scientific Tracks Abstracts: Arch Clin Microbiol

Abstract:

Liver texture was comparatively assessed in 20 specimens of carp after experimental exposure to two PFOA dosages (10 exposed to 200 ng l-1, 10 exposed to 2 mg l-1) for 56 days and in 10 unexposed (Ctr) specimens. Grayscale differential box counting (mass fractal dimension, DM and lacunarity, λ [Lac]; FracLac plugin in ImageJ package; Fig. 1) was adopted on images previously assessed for texture by means of MaZda software. Linear Discriminant Analysis on grayscale box counting data failed to discriminate with confidence between treated and untreated fish (40% of cases correctly classified: sensitivity of 69.0%, specificity of 52.6%) with particular regard to low dosage group (only 10% of Ctr cases correctly classified). Nevertheless, referring to the cumulative Redundancy Analysis ordination space (Fig. 2), the more the liver PFOA content augmented, the more the number of cells positive to Proliferating Cell Nuclear Antigen (PCNA), the liver mass, DM and λ (hence gray level pattern complexity) increased and Sum Entropy (hence the disorder of a vector from the gray level cooccurrence matrix) decreased. To date the exact biological implication of such correlations is not known, with lacunarity increase possibly due to hepatocyte degeneration (increased vacuolisation), though it surely deserves further investigation in order to use grayscale box counting with confidence in biomarker evaluation in course of environmental monitoring.

Biography :

Maurizio Manera has completed his PhD in Veterinary Pathology at Bologna University and MSc in Environmental Science at L’Aquila University. He is an Assistant Professor in Image Analysis in Histopathology and in Biomarkers in Environmental Monitoring. He has published 74 papers in indexed reputed journals and has been serving as an Editorial Board Member of reputed journals.

E-mail: mmanera@unite.it