Annals of Clinical and Laboratory Research

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Molecular study of Panton-Valentine leukocidin gene and MecA among Staphylococcus aureus isolated from hospital acquired infections and community acquired infections

4th Edition of International Conference on Clinical Chemistry & Molecular Diagnostics
April 15-16, 2019 | Paris, France

Maysaa El Sayed Zaki and Sameh Salama

Mansoura Faculty of Medicine, Egypt AlAzhar University, Egypt

Posters & Accepted Abstracts: Ann Clin Lab Res


In the present study, the aim was to define the prevalence of Panton Valentine leukocidin gene and MecA genes among community acquired infection (CAI) and hospital acquired infections (HAI) due to S aureus strains. The study was performed on one hundred ninety six S aureus strains isolated from clinical samples from hospital acquired infections obtained from HAI or CAI. S aureus isolates were subjected to molecular study for MecA gene and PVL by multiplex polymerase chain reaction (multiplex-PCR). PVL gene was significantly detected in higher frequency in CAI (41.9%), P=0.0001 compared to HAI (16.1%). However, MecA gene was detected significantly in HAI (23.6%), P=0.02 compared to CAI (10.8%), The majority of MRSA strains had PVL gene (66.7%). The findings of the present study support the hypothesis that PVL gene was significantly associated with community acquired infections. However, the combined presence of MecA gene and PVL were also common in S aureus isolated from hospital acquired infections. PVL gene was prevalent in wound infections. Further studies are required to determine if PVL gene can be used as a distinctive marker of CAI infections in Egypt.

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