Archives of Clinical Microbiology

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Prevalence of microorganisms and their antimicrobial susceptibility profile in blood samples from an university hospital from Vitoria, Brazil

August 23-24, 2017 | Toronto, Canada

Moraes Rodrigo, Alexandre Coelho Guimar?£es, Carla Coradini de Mattos Siqueira, Thays Furtado Dias da Mata, Rodrigo Pratte Santos and Carolina Frizzera Dias

Santa Casa de Miseric?³rdia de Vit?³ria, Brazil

Posters & Accepted Abstracts: Arch Clin Microbiol


Due to the elevated number of nosocomial infections and its relation with intra-hospital morbidity and mortality levels, it is crucial to evaluate the responsible agents in order to improve patientsâ?? care. This is an observational, retrospective and transversal study performed at the Hospital da Santa Casa de Miseric?³rdia de Vit?³ria, involving 511 patients infected between July 2014 and June 2016. Data on blood culture samples were collected from the database of the Hospital Infection Control Committee (CCIH). For blood culture, sheep blood agar (SBA), chocolate agar and MacConkeyâ??s agar (MAC), after culture in an automated blood culture system were used. Microbial identification and susceptibility profile evaluation were performed using the MicroScan auto SCAN-4 (Beckman Coulter?®) automated system. The study describes four bacterias in detail: Acinetobacter spp., Klebsiella pneumoniae, Pseudomonas aeruginosa and S. aureus. The study found that the least active antimicrobials against S. aureus were nalidixic acid and ampicillin, while the most active were amikacin and cefazolin. Secondly, the least active antimicrobials against Acinetobacter spp. were aztreonam and ertapenem, whereas the most active were polymyxin and rifampicin. Furthermore, the least active antimicrobials against K. pneumoniae were ampicillin and ampicillin/sulbactam, while the most active were amikacin and cefoxitin. Finally, the least active antimicrobials against P. aeruginosa were ceftriaxone and cefotaxime, while the most active were erythromycin and polymyxin. Physicians often have difficulty in establishing the susceptibility profile of the etiologic agent of an infection. Thus, knowledge about antimicrobial resistance from the hospital is fundamental, improving clinical management of patients.

Biography :

Moraes Rodrigo has completed his graduation in Pharmacy from Emescam College (2004), Post-graduated in Microbiology from PUC University (2006) and Master’s in Biological Sciences (Microbiology) from Federal University of Minas Gerais (UFMG) (2008). He is currently the Coordinator of the Biomedicine Course at PIO XII Faculty and Professor of Microbiology, Cell Biology and Biochemistry, and Professor of Microbiology at Emescam (School of Sciences of Santa Casa de Misericórida de Vitória, ES). He is also a member of the Research Ethics Committee and of the Medical Course Collegiate from Emescam.