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Archives of Clinical Microbiology

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The BceABRS four-component system essential for bacitracin sensing and response is required for biofilm formation and virulence of Streptococcus mutans

Annual Conference on MICROBIAL PATHOGENESIS, INFECTIOUS DISEASE, ANTIMICROBIALS AND DRUG RESISTANCE
August 23-24, 2017 | Toronto, Canada

Yung-Hua Li, David Ropson, Anton Dugandzic and Xiao-Lin Tian

Dalhousie University, Canada

Posters & Accepted Abstracts: Arch Clin Microbiol

Abstract:

Streptococcus mutans is a primary etiological agent of dental caries worldwide. Natural life of S. mutans in dental biofilms often faces life-threatening insults, such as killing by antibiotics or innate defense molecules produced by competing species or by the host. How such insults affect physiology and virulence of S. mutans is poorly understood. In this study, we explored this question by analyzing the effects of sub-MIC concentrations of bacitracin and ??-defensin 3 on S. mutans. Microarray analysis showed that both bacitracin and ??-defensin 3 induced differential expression of subsets of genes that were largely regulated by the BceABRS fourcomponent system. The results were further confirmed by examining gene expression profiles of selected genes or genetic loci using qRT-PCR. We then examined the effects of gene deletion of bceRS on the peptide antibiotics and virulence. The results showed that a deletion of bceRS resulted in a mutant that was sensitive to bacitracin or ??-defensin 3. Introduction of a wild copy of becRS in trans (complementation) restored the wild type phenotype of the mutant. In particular, both peptide antibiotics at a sub-MIC induced biofilm formation in the parent but not in the mutant. A competitive fitness analysis showed that the mutant was unable to compete with the parent for co-existence in duel-strain mixed cultures in the presence of bacitracin. In conclusion, the BceABRS four-component system controls a regulon that is required for sensing, response and resistance to bacitracin and ??-defensin. This system may play an important role in adaptation and virulence expression of S. mutans in dental biofilms.

Biography :

Yung-Hua Li received his Doctorate in Molecular Microbiology at University of Manitoba. Following his Post-doctoral fellowships in the University of British Columbia and University of Rochester, NY, he worked as a Scientist in the University of Toronto, with his research focus on molecular dissection of microbial biofilms. In 2004, he joined the Faculties of Dentistry and Medicine at Dalhousie University, where he has been directing a research team on genetic analyses of bacterial biofilms, biofilm ecology and pathogenesis.