Darakhshanda Neelam*, S.A. Husain, Tanveera Tabasum and M. Maqbool lone
Introduction: Medicinal plants play a central role in the healthcare system of the large proportions of the world’s population. Plant derived compounds are an important source of several clinically useful anticancer agents. Cytotoxicity screening models are the preliminary methods for the selection of active extracts against cancer.
Materials and methods: A comprehensive study was carried out to determine the Cytotoxic activity of active extracts of the plant-P. vulgaris, T. buccatta against human lung cancer (A549) cell line at eight different concentration to determine the IC50 (50% growth inhibition) by MTT assay including morphological study by fluorescence microscopy, Apoptosis by Annexin V, caspase 3-7 assay and levels of expression of genes. Each sample was assayed in triplicate and control samples include cells without plant extracts.
Results: Results demonstrate that the percentage of growth inhibition increases with increasing concentrations of test compounds. The P. vulgaris extracts induced significant cytotoxic effects on the A549 cancer cell line and these effects were stronger than the other selected plant extracts reflecting a promising way of chemotherapy to counter the spread of non- small cell lung cancer.
Conclusion: Extract which exhibit substantial antipoliferative and apoptic activity may represent a source for novel natural anticancer entities.
Published Date: 2023-10-31; Received Date: 2023-10-10
