Health Science Journal

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Protein Microarray Analysis of Soluble Biomarkers in Fertile and Postmenopausal Women in Relation to Obesity, Bone Status and Blood Cell Populations

Mira Horvathova, Michaela Szabova, Kornelia Stefikova, Jana Tulinska, Zora Krivosikova, Viera Spustova, Aurelia Lískova and Martin Gajdos

Objective: The purpose of the present study was to quantify serological biomarkers, namely adiponectin, leptin, E-selectin, ICAM-1 (intercelluar cell adhesion molecule-1), IGF-1 (insulin-like growth factor 1), MCSF (macrophage colony-stimulationg factor), IL-6, IL-10 and IL-17), in fertile and postmenopausal women. The relationship of these parameters to obesity and bone status has been analysed. Methods: 96 fertile and 107 postmenopausal women were enrolled in this study. The detection of multiple biomarkers, cytokines, growth factors, adhesion molecules and others, was performed by protein microarray. Results: Adipopnectin was up-regulated in postmenopausal control and ICAM-1 in postmenopausal obese women when compared to fertile obese women (p<0.05). Significant negative correlation was between IL-6 and age (r=-0.177, p<0.05). The soluble form of ICAM-1 was higher in women with osteopenia, but not with osteoporosis, in comparison to the control group. IL-6 was decreased in women affected by osteoporosis. Conclusion: In context to obesity, bone status and ageing, we have found alterations in adiponectin, ICAM-1, and IL-6 parameters in postmenopausal women. We identified several statistically significant correlations between soluble biomarkers and cell populations (e.g. between IGF-1 and B-lymphocytes; IL-6 and dendritic cells; IL-10 and naive T cells, etc.).