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The Effects on Cortisol, Glucose and Lysozyme Activity in Different Concentration of Formaldehyde in Rainbow Trout


In this study fish diseases and therapeutic purposes prophylactic widely used formalin (CH2O)'s in rainbow trout (Oncorhynchus mykiss) cortisol, glucose and their effects on lyso-zyme activity were examined. October-November 2009 the researches have been carried out among the 60 rainbow trout weighing approximately 180 g were used. Replications in the ex-periment conducted of formalin 250 mg/L (60 minutes), 500 mg/L (45 minutes) concentrations were applied. For each group of 20 fish, cortisol, serum glucose and lysozyme activity was used for measurements. Cortisol analysis ECLIA (Electro chemiluminescence Immun Assay) method, lysozyme activity diffusion agar (agar plate, lysoplate) with the method of analysis and glucose were determined by spectrophotometric methods. The data obtained in the experi-ment of the SPSS package statistical program to evaluate all the data using analysis of variance (ANOVA) was applied and the group mean differences between LS Means JMP Student's T test multiple comparison test with significance level according to p