This study was carried out to determine the spermatological characteristics in flounder (Plat-ichthys flesus luscus Pallas, 1814). In the spawning season, semen was collected by abdominal massage from 10 male. In collected milts; volume, motility, duration of motility, spermatocrit ratio, concentration and pH were determined. Furthermore, body weight and total length were measured and correlations between spermatological characteristics and these parameters were investigated. In the semen of flounder, milt volume (ml), motility (%), duration of motility (min), spermatocrit ratio, concentration (x 109 /ml), and pH values were found as mean 0.2 ± 0.04, 88.0 ± 13.80, 25.4 ± 4.20, 92.6 ± 4.3, 2.8 ± 0.72 ve 6.9 ± 0.10, respectively. It was found that there were positive correlations between body weigth and total length, and volum (P < 0.01), but negatif correlation between total length and duation of motility (P 0.05), and be-tweem density and spermatocrit (P 0.05).